Plot

After the events of Resident Evil: Extinction, the Alice clones (Milla Jovovich) attempt to kill Albert Wesker (Shawn Roberts) at his base in Japan. All present clones are killed in an explosion, as Wesker escapes in an aircraft. The original Alice ambushes him, but Wesker injects her with a serum that neutralizes the T-virus in her system, eliminating her superhuman powers and making her human again. He reveals that his T-Virus powers, have surpassed her own, but before he can kill her, the aircraft crashes and Alice emerges from the wreckage.

Six months later, Alice follows a repeating emergency broadcast from a survivors' safe haven known as "Arcadia" in Alaska. After many fruitless months searching for Arcadia and finding no survivors along the way, including her friends Claire Redfield (Ali Larter) and K-Mart (Spencer Locke), Alice begins to lose hope, believing herself to be the last surviving human on the planet. Stopping on an abandoned beach, Alice finds the helicopter taken by Claire and the others when they and Alice parted ways. A crazed Claire, under the influence of an Umbrella Corporation device attached to her chest, attacks her. Alice removes the device, finding that it has damaged Claire's memory, which she slowly regains.

Flying a two-person plane, they enter the ruins of Los Angeles and find a small group of survivors, led by Luther West (Boris Kodjoe) living in a maximum security prison, surrounded by the undead. Alice crash lands the plane on the roof of the prison and learn that Arcadia is not a fixed place but actually a cargo tanker traveling along the coast. Since the plane cannot take more than two, Alice and the survivors try to figure out a means to make it to Arcadia together. The survivors have been keeping watch on a prisoner, Chris (Wentworth Miller), whom they found locked in a maximum security cell when they arrived. He says that he is a soldier who fought the early outbreak, but was locked up by prisoners as a cruel joke when the mission failed and knows a way to reach the coast, and thereby Arcadia, but will not reveal it unless he is released.

They do not believe him, and keep him locked up until the undead manage to tunnel through the sewer ways and up into the prison, and a giant axe wielding Nemesis monster starts to break down the front gate; Alice and survivors are out of time to find a means to reach Arcadia so they decide to free Chris and use his escape route. He recognizes Claire and reveals himself as her brother, though she does not remember him. Chris' proposed method, a stored military vehicle, is legitimate, but the vehicle is not operational and they are left with no other option but to use the tunnel to escape through the sewers, and storm drains which empty at the coast. The group fights their way out — Alice, Claire, and Chris manage to escape into the sewers.

They continue to Arcadia, finding the ship completely functional, but abandoned. Exploring its inner depths, they realize that it is a trap set by Umbrella to lure survivors to the ship to conduct experiments on them. Claire now remembers what happened to her group when they arrived to Alaska: Umbrella ambushed their group when they landed on the beach, attaching the devices to them, though she managed to escape. They release the survivors, among them K-Mart, and Alice continues deeper into the ship, finding escape helicopters and a purging bomb. Deeper inward, she finds Wesker, who explains the price of his survival, is a war within himself for control against the T-Virus. By assimilating Alice, the only individual to bond successfully with the T-virus, he might be able to gain full mastery of it. At this point the crew of the ship had abandoned Wesker, terrified by his cannibalism.

Chris, Claire, and Alice battle the super-powered Wesker and seemingly kill him with help from a recovered K-Mart. Wesker does not die, but regenerate and escapes on a helicopter. He activates the purge bomb on the Arcadia in attempt to destroy the ship and kill the remaining survivors. Anticipating his actions Alice had hidden the bomb on board the helicopter, which is destroyed by the explosion. Alice, Claire, and Chris watch the destruction from the deck of the Arcadia.

Alice resolves to turn Arcadia into a real safe haven, and broadcasts its message for any other survivors. As Claire, Chris, and Alice decide how to proceed with all the survivors, they see an approaching fleet of Umbrella assault helicopters.

Cast

Main article: List of Resident Evil characters

* Milla Jovovich as Alice: A former Umbrella Corporation security officer whose exposure to the T-Virus granted her superhuman abilities. Since her escape from Raccoon City, she has been hunted by Umbrella, who intends to use her DNA in order to control the virus' mutation. Alice seeks to "settle her score" with Umbrella and its elusive chairman, Albert Wesker.[3][4]
* Ali Larter as Claire Redfield: Chris' younger sister. She led a convoy of survivors who encountered Alice in the previous film. In Afterlife, Claire mysteriously finds herself alone in the woods and suffering from amnesia.[5]
* Wentworth Miller as Chris Redfield: Claire's older brother. He is working with an unnamed military organization combating the infected. Chris and his unit were using a Los Angeles prison as a base of operations, before it was overrun and he was imprisoned.[6]
* Shawn Roberts as Albert Wesker: The Chairman of the Umbrella Corporation. He has been tracking Alice in order to discover means by which the T-Virus mutation can be controlled. His office is located in Tokyo, Japan, and he communicates with the company's other branches using holographic technology. Similar to his video game counterpart, Wesker possesses superhuman strength, speed, and regenerative capabilities.[6]
* Spencer Locke as K-Mart: Named after the department store she was discovered hiding in, K-Mart was a member of Claire Redfield's convoy of survivors in the previous film.[6]
* Boris Kodjoe as Luther West: A former professional basketball player and the leader of the survivors of Los Angeles. Kodjoe trained for six weeks to perform his own stunts. He dislocated his knee in the course of filming while trying to kick in a gate.[7]
* Kim Coates as Bennett: A big-shot film producer who is strongly disliked by the group, save for Kim. He is a traitorous member of Luther's group.
* Kacey Barnfield as Crystal: A junior champ in swimming in her high school days. She came to Los Angeles to work as an actress.
* Norman Yeung as Kim Yong: Bennet's former intern before the virus outbreak.
* Sergio Peris-Mencheta as Angel Ortiz[8]
* Sienna Guillory as Jill Valentine: A former STARS operative who assisted Alice and escaped Raccoon City in Resident Evil: Apocalypse. Guillory, who portrayed the character in Apocalypse, reprises her role in the film.[9] She features briefly in a scene shown during the credits, setting up a potential sequel.
* Ray Olubowale as The Axeman : A Nemesis type monster (Executioner Majini). The Axeman first appears in the streets of Los Angeles, dragging an enormous axe behind him. He eventually makes his way to the prison compound where Alice and the other survivors have taken refuge, and breaks down the main gate, allowing hordes of zombies to infiltrate the facility.